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mouse anti il10rb antibody  (R&D Systems)


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    R&D Systems mouse anti il10rb antibody
    Mouse Anti Il10rb Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il10rb/pm41932922-326-18-24?v=R%26D+Systems
    Average 94 stars, based on 12 article reviews
    mouse anti il10rb antibody - by Bioz Stars, 2026-07
    94/100 stars

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    Identification of IGSF8 and IL10RB as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: An Integrative Proteotranscriptomics Approach Reveals New ADAM9 Substrates and Downstream Pathways

    doi: 10.1016/j.mcpro.2026.101538

    Figure Lengend Snippet: Identification of IGSF8 and IL10RB as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.

    Article Snippet: Antibodies used in this study include rabbit anti-ADAM9 (CST, 4151, 1:1000 dilution), mouse anti-ALCAM (Santa Cruz, 74558, 1:200 dilution), mouse anti–interleukin 10 receptor subunit beta (IL10RB) (R&D Systems, MAB874, 5 μg/ml), rabbit anti–Forkhead Box O3 (FOXO3) (CST, 2497, 1:1000 dilution for Western blotting, and 1:100 dilution for immunocytochemistry [ICC]), rabbit anti–immunoglobulin superfamily member 8 (IGSF8) (Sigma, 011917, 1:500 dilution), horseradish peroxidase (HRP)–conjugated rabbit anti-mouse (CST, 7076, 1:2000 dilution), HRP-conjugated goat anti-rabbit (CST, 7074; 1:2000 dilution), HRP-conjugated mouse anti-β-actin antibody (CST, 12262, 1:2000 dilution), and Alexa Fluor 594–conjugated goat anti-rabbit antibody (Thermo Fisher, A-11012, 1:1000 dilution for ICC).

    Techniques: Transfection, Expressing, Construct, Western Blot, Two Tailed Test

    Relative mRNA expression of the receptor subunits IFNAR1, IFNAR2, IFNLR1 and IL10RB in SW780 and RT4 cells. RNA was isolated from the individual cell lines, reverse-transcribed into cDNA and then analysed for expression of the IFN RS by qPCR. The expression levels of the individual RS were normalised to the geometric mean of the reference genes TBP and HPRT1

    Journal: Journal of Cancer Research and Clinical Oncology

    Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

    doi: 10.1007/s00432-025-06409-1

    Figure Lengend Snippet: Relative mRNA expression of the receptor subunits IFNAR1, IFNAR2, IFNLR1 and IL10RB in SW780 and RT4 cells. RNA was isolated from the individual cell lines, reverse-transcribed into cDNA and then analysed for expression of the IFN RS by qPCR. The expression levels of the individual RS were normalised to the geometric mean of the reference genes TBP and HPRT1

    Article Snippet: The following Taqman assays (Thermo Fisher Scientific) were used: HPRT1 (Hs02800695_m1), IFI44 (Hs00197427_m1), IFIT1 (Hs01675197_m1), IFIT2 (Hs00533665_m1), IFNAR1 (Hs01066118_m1), IFNAR2 (Hs01022060_m1), IFNLR1 (Hs00417120_m1), IL10RB (Hs00175123_m1), IRF9 (Hs00196051_m1), ISG15 (Hs00192713_m1), MX1 (Hs00895608_m1), TBP (Hs00427620_m1).

    Techniques: Expressing, Isolation, Reverse Transcription